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Sequence
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All samples and primers must be dissolved in distilled water, and must be at the standard concentrations listed in the table below
For information about ordering a Premix sequencing services, click here
| Type of DNA | Amount | Concentration |
| PCR Fragments100-500bp | 30ng/reaction* | 10ng/μl |
| PCR Fragments500-1000bp | 60ng/reaction* | 20 ng/μl |
| PCR Fragments>1000bp | 200ng/reaction* | 25ng/μl |
| DS Plasmid DNA (3-10Kb) | 600ng/reaction* | 100-300 ng/μl |
| DS Plasmid DNA (10-20Kb) | 1.2µg/reaction* | 100-500 ng/μl |
| BAC/Cosmid DNA>20kb | 1.2µg/reaction* | 100-500 ng/μl |
- Please provide Tm of your custom primer
- Primer concentration: 5 pmol/μl (10pmol/reaction)
Guidelines for optimal custum primer design:
- Length of 18-25 bases
- GC content of 50-65%
- Tm of 55-60oC
- No secondary priming sites
- No dimerization capability
- No significant hairpins (> 3 bp)
|
Name |
Sequence |
|
M13-20F |
5'-TGT AAA ACG ACG GCC AGT-3' |
|
M13-REV |
5'-AAC AGC TAT GAC CAT GAT TA-3' |
|
PcDNA3.1-F |
5' -CTC TGG CTA ACT AGA GAA C-3' |
|
pcDNA3.1 REV |
5'- TAG AAG GCA CAG TCG AGG -3' |
|
pET upstream |
5'- ATG CGT CCG GCG TAG A -3' |
|
pET-24a |
5' -GGG TTA TGC TAG TTA TTG CTC AG-3' |
|
pET-F |
5' -TCA TCA TTC TTC TGG TCT G-3' |
|
pGEX For |
5' -GGG CTG GCA AGC CAC GTT TGG TG-3' |
|
pGEX Rev |
5'-CCC GGA GCT GCA TGT GTC AGA GG-3' |
|
PUC-REV |
5'-AGC GGA TAA CAA TTT CAC AC-3' |
|
SP6 |
5'-CCA AGC TAT TTA GGT GAC AC-3' |
|
SP6 (pGEM) |
5'-TAT TTA GGT GAC ACT ATA G-3' |
|
SP6A (for pcDNA3) |
5'-GCA TTT AGG TGA CAC TAT AG -3' |
|
T3 |
5'-GCG CAA TTA ACC CTC ACT AAA G-3' |
|
T7 |
5'-TAA TAC GAC TCA CTA TAG GG-3' |
|
T7 Terminator |
5'- GCT AGT TAT TGC TCA GCG G-3' |
This information will contribute to the optimization of the sequence conditions
Pay attention! The size you inserted is not suitable for plasmids but for PCR amplicons only. Unless you change the size it will be submitted as a PCR amplicon.
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